glxB [2019-07-08 09:05:16]

glyoxalase II

locus: BSU32660

pI: 4.61

mw: 14.30 kDa

protein length: 127 aa

gene length: 384 bp

function: detoxification of methylglyoxal

product: glyoxalase II

essential: no

synonyms: yurT

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 3,354,551 3,354,934

The protein

Catalyzed reaction/ biological activity

• S-lactoyl-bacillithiol (BSH) - D-lactate + BSH [Pubmed|24330391]

Structure

• [PDB|4G6X] (from Catenulispora acidiphila, 53% identity)

Expression and Regulation

Operons

• genes: [gene|85E5D3211F27DA64C4E829385C6A08F4A4A2F874|yuzN]-[gene|0A244A61A9A484F0BF083C918AE5A2C6A3F8157E|glxB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B582 (yurT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1581 NBRP B. subtilis, Japan]
• BKE32660 ([gene|0A244A61A9A484F0BF083C918AE5A2C6A3F8157E|glxB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGTTTTTCCCTCCTT, downstream forward: _UP4_TAACCGATTCAGTCGCGTCC
• BKK32660 ([gene|0A244A61A9A484F0BF083C918AE5A2C6A3F8157E|glxB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGTTTTTCCCTCCTT, downstream forward: _UP4_TAACCGATTCAGTCGCGTCC

References

• 24330391